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KMID : 1011620110270040067
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Korean Journal of Food and Cookey Science
2011 Volume.27 No. 4 p.67 ~ p.73
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Neuroprotective Effects of Extracts from Diospyros kaki L. Peel
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이미라:Lee Mi-Ra
문성희:Moon Seong-Hee/최애란:Choi Ae-Ran/이승철:Lee Seung-Cheol/안광환:Ahn Kwnag-Hwan/박해룡:Park Hae-Ryong
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Abstract
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This study was performed to assess the neuroprotective effects of methanolic extracts from sweet persimmon peel (PPE) against glutamate-induced cytotoxicity in hybridoma N18-RE-105 cells. The neuroprotective effects of PPE in N18-RE-105 cells were measured using the MTT reduction assay, LDH release assay, and phase-contrast microscopy. The results of the MTT reduction assay showed that treating cells with 500 μg/mL PPE resulted in cell viability of 66.9%. Additionally, the morphological changes and the results of the LDH release assay showed that glutamate-induced damage to nerve cells was strongly inhibited by PPE. GSH content of N18-RE-105 cells was 3.5 μM compared to that of the control, whereas pretreatment with 500 μg/mL PPE increased GSH content by 4.7 μM. PPE was fractionated with hexane, and that layer had the highest neuroprotective effects in glutamate-stressed N18-RE-105 cells. In conclusion, our data showed that glutamate potentiated the effects of N18-RE-105 cell death by a mechanism involving oxidative stress. Therefore, PPE may be a potential candidate for prevention and therapy of neurodegenerative diseases.
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KEYWORD
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neuroprotective effect, sweet persimmon, glutamate, N18-RE-105 cells, glutathion
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